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. 2006 Apr 15;20(8):939–953. doi: 10.1101/gad.1388706

Figure 5.

Figure 5.

(A) A specific protein complex assembles on the 73-nt TDE. Western blot analysis of the protein complexes formed on biotinylated RNA encoding full-length 3′ UTR (1–612) or the 73-nt (465–537) or on RNA lacking the TDE (1–407). The complexes formed on RNA–streptavidin beads were resolved on SDS-PAGE and immunoblotted to nitrocellulose membrane. The blots were probed with antibodies to various proteins. (B) The UV-cross-linked proteins were immunoprecipitated by anti-HuR and anti-C1/C2 antibodies. The proteins cross-linked to the full-length 3′ UTR (1–612) or to the 73-nt binding site were incubated with various antibodies and the immunoprecipitated complexes resolved by SDS-PAGE and visualized by autoradiography.