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Normal recruitment of 3′-processing factors requires functional Rat1. (A) ChIP of pol II, Pcf11, and Rna15 on GAL1-ADH4 in rat1-1 transformed with control vector (DBY 768). (Lanes 6,8) Note recruitment of Pcf11 and Rna15 is reduced by inactivation of Rat1 at 37°C. (B) ChIP as in A of rat1-1 transformed with the wild-type RAT1 plasmid (DBY754). (Lanes 6,8) Note recruitment of Pcf11 and Rna15 at 37°C is partially restored relative to A. HMR-negative controls were run on separate gels.
