TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristics and origin | Source and/or reference |
|---|---|---|
| Strains | ||
| Escherichia coli Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(araA-lei)7697 galU galK rpsL endA1 nupG | Invitrogen GmbH, Karlsruhe, Germany |
| Actinomadura sp. strain E6 | Rubber-degrading wild type, isolated from food residues of animal husbandry in Egypt (clear zone forming) | This study |
| Gordonia polyisoprenivorans VH2 | Rubber-degrading wild type (adhesive) | DSM 44266 (1) |
| Gordonia westfalica Kb1 | Rubber-degrading wild type (adhesive) | DSM 44215T (11) |
| Nocardia farcinica E1 | Rubber-degrading wild type, isolated from top soil from Egypt (adhesive) | This study |
| Nocardia farcinica E2 | Rubber-degrading wild type, isolated from sandy soil from Egypt (adhesive) | This study |
| Nocardia farcinica E3 | Rubber-degrading wild type, isolated from soil of a gas station in Egypt (adhesive) | This study |
| Streptomyces lividans TK23 | 9 | |
| Thermomonospora curvata E4 | Rubber-degrading wild type, isolated from food residues of animal husbandry in Egypt (clear zone forming) | This study |
| Thermomonospora curvata E5 | Rubber-degrading wild type, isolated from compost in Germany (clear zone forming) | This study |
| Nocardia farcinica S3 | Rubber-degrading wild type, isolated from sandy soil from Egypt (adhesive) | This study |
| Nocardia farcinica I7 | Rubber-degrading wild type, isolated from sandy soil from Cambodia (adhesive) | This study |
| Plasmids | ||
| pBluescript SK(−) | AprlacPOZ′, 2.95 kb | Stratagene, San Diego, Calif. |
| pIJ702 | 9 | |
| pIJ702::lcpNFE1 | Contains an lcp homologue from N. farcinica E1 | This study |