TABLE 1.
Blast results of ORFs identified on the metagenome cosmid clones
| ORF | Size (aaa) | Possible function and GenBank accession no. | Microorganism | % Identity (no. of similar aa/total no.) |
|---|---|---|---|---|
| pCosCE1 | ||||
| ORF001 | 271 | Hypothetical protein, NP_669416 | Yersinia pestis | 48 (123/256) |
| estCE1 | 389 | β-Lactamase class C, ZP_00486659 | Burkholderia pseudomallei | 51 (196/378) |
| ORF003 | 310 | Probable regulator, NP_103137.1 | Mesorhizobium loti | 42 (124/293) |
| ORF004 | 224 | Putative transcriptional regulator, tetR family, ZP_00235387 | Bacillus cereus | 40 (78/191) |
| ORF005 | 385 | Arabinose efflux permease, ZP_00136708 | Pseudomonas aeruginosa | 45 (171/380) |
| ORF006 | 379 | β-Lactamase class C, CAB69829.1 | Serratia marcescens | 77 (302/378) |
| pCosCS-A3 | ||||
| ORF001 | 359 | Aminoglycoside phosphotransferase, ZP_00579208.1 | Sphingopyxis alaskensis | 79 (280/353) |
| ORF002 | 253 | Oxidoreductase, NP_421870.1 | Caulobacter crescentus | 73 (186/254) |
| ORF003 | 265 | Short-chain dehydrogenase/reductase ZP_00579206.1 | Sphingopyxis alaskensis | 81 (216/265) |
| estA3 | 396 | β-Lactamase, ZP_00579205.1 | Sphingopyxis alaskensis | 59 (236/396) |
| ORF005 | 301 | Protein of unknown function, ZP_00577139.1 | Sphingopyxis alaskensis | 57 (158/277) |
| ORF006 | 171 | Similar to aspartyl protease, ZP_00577809.1 | Sphingopyxis alaskensis | 40 (49/120) |
| ORF007 | 153 | Putative protein disulfide isomerase, ZP_00375704.1 | Erythrobacter litoralis | 43 (58/133) |
| ORF008 | 108 | Hypothetical protein, ZP_00567441.1 | Frankiasp. | 28 (29/101) |
a
aa, amino acids.