TABLE 2.
Specific enzyme activities of EstCE1 and EstA3
| Substrate | Sp act (U/mg)a
|
|
|---|---|---|
| EstCE1 | EstA3 | |
| pNP-acylesters | ||
| Butyrate (C4) | 31.1 | 513.6 |
| Valerate (C5) | 22.9 | 425.7 |
| Caproate (C6) | 7.4 | 425.7 |
| Octanoate (C8) | 1.0 | 3.6 |
| Decanoate (C10) | 0.9 | 0 |
| Palmitate (C16) | 0.0 | 0 |
| Triglycerides | ||
| Triacetin (C2) | 26.7 | 147.5 |
| Tripropionin (C3) | 33.3 | ND |
| Tributyrin (C4) | 54.7 | 167.5 |
| Tricaprylin (C8) | 0 | ND |
| Trilaurin (C12) | 0 | ND |
| Vinyl acids | ||
| Vinyl acetate (C2) | 13.3 | 107.5 |
| Vinyl propionate (C3) | 13.3 | 380.0 |
| Vinyl butyrate (C4) | 16.0 | 387.5 |
| Vinyl caproate (C6) | 0 | 237.5 |
| Vinyl caprylate (C8) | 0 | 155.0 |
| Vinyl laurinate (C12) | 0 | 7.5 |
a
ND, not determined.