TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Reference or source |
|---|---|---|
| Bacterial strains | ||
| E. coli JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F−[traD36 proAB+lacIqlacZΔM15] | 35 |
| E. coli DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 35 |
| Sphingomonas sp. strain KA1 | Car+ | 9 |
| Plasmids | ||
| pUC119 | AprlacZ, pMB9 replicon, M13IG | 35 |
| pT7Blue(R) | AprlacZ | Novagen |
| pUKAcarAaI | Apr; pUC119 with 1.2-kb SphI-XbaI DNA fragment containing the carAaI gene of strain KA1 | This study |
| pUKAcarAaII | Apr; pUC119 with 1.2-kb SphI-XbaI DNA fragment containing the carAaII gene of strain KA1 | This study |
| pUKAcarAcI | Apr; pUC119 with 0.3-kb XbaI-KpnI DNA fragment containing the carAcI gene of strain KA1 | This study |
| pUKAcarAcII | Apr; pUC119 with 0.3-kb XbaI-KpnI DNA fragment containing the carAcII gene of strain KA1 | This study |
| pUKAfdxI | Apr; pUC119 with 0.3-kb XbaI-KpnI DNA fragment containing the fdxI gene of strain KA1 | This study |
| pUKAfdrI | Apr; pUC119 with 1.2-kb KpnI-EcoRI DNA fragment containing the fdrI gene of strain KA1 | This study |
| pUKAfdrII | Apr; pUC119 with 1.2-kb KpnI-EcoRI DNA fragment containing the fdrII gene of strain KA1 | This study |
| pUKA248 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaI) from pUKAcarAaI and 0.3-kb XbaI-KpnI fragment (carAcI) from pUKAcarAcI | This study |
| pUKA249 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaI) from pUKAcarAaI and 1.2-kb KpnI-EcoRI fragment (fdrII) from pUKAfdrII | This study |
| pUKA250 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaI) from pUKAcarAaI and 1.2-kb KpnI-EcoRI fragment (fdrI) from pUKAfdrI | This study |
| pUKA253 | Apr; pUC119 with 1.5-kb SphI-KpnI fragment (carAaI and carAcI) from pUKA248 and 1.2-kb KpnI-EcoRI fragment (fdrII) from pUKAfdrII | This study |
| pUKA254 | Apr; pUC119 with 1.5-kb SphI-KpnI fragment (carAaI and carAcI) from pUKA248 and 1.2-kb KpnI-EcoRI fragment (fdrI) from pUKAfdrI | This study |
| pUKA255 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaII) from pUKAcarAaII and 0.3-kb XbaI-KpnI fragment (carAcII) from pUKAcarAcII | This study |
| pUKA256 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaII) from pUKAcarAaII and 1.2-kb KpnI-EcoRI fragment (fdrII) from pUKAfdrII | This study |
| pUKA257 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaII) from pUKAcarAaII and 1.2-kb KpnI-EcoRI fragment (fdrI) from pUKAfdrI | This study |
| pUKA260 | Apr; pUC119 with 1.5-kb SphI-KpnI fragment (carAaII and carAcII) from pUKA255 and 1.2-kb KpnI-EcoRI fragment (fdrII) from pUKAfdrII | This study |
| pUKA261 | Apr; pUC119 with 1.5-kb SphI-KpnI fragment (carAaII and carAcII) from pUKA255 and 1.2-kb KpnI-EcoRI fragment (fdrI) from pUKAfdrI | This study |
| pUKA263 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaI) from pUKAcarAaI, 0.3-kb XbaI-KpnI fragment (fdxI) from pUKAfdxI, and 1.2-kb KpnI-EcoRI fragment (fdrI) from pUKAfdrI | This study |
| pUKA265 | Apr; pUC119 with 1.2-kb SphI-XbaI fragment (carAaII) from pUKAcarAaII, 0.3-kb XbaI-KpnI fragment (fdxI) from pUKAfdxI, and 1.2-kb KpnI-EcoRI fragment (fdrI) from pUKAfdrI | This study |
| pUCARA | Apr; pUC119 with 5.3-kb EcoRI DNA fragment containing carAaAaAc (ORF7) carAd genes of Pseudomonas resinovorans strain CA10 | 36 |
a
Car+ represents an ability to grow on CAR as the sole source of carbon, nitrogen, and energy. Apr represents resistance to ampicillin.