TABLE 3.
Carbazole conversion by E. coli expressing a putative CARDO component(s)
| Plasmid used | Protein(s) expressed in E. coli JM109
|
Conversion ratio (%)a | ||
|---|---|---|---|---|
| Oxygenase | Ferredoxin | Reductase | ||
| pUKAcarAaI | CarAaI | 0.090 (±0.030) | ||
| pUKA248 | CarAaI | CarAcI | 0.34 (±0.21) | |
| pUKA250 | CarAaI | FdrI | 0.19 (±0.089) | |
| pUKA254 | CarAaI | CarAcI | FdrI | 96 (±1.4) |
| pUKA263 | CarAaI | FdxI | FdrI | 11.7 (±6.9) |
| pUKA249 | CarAaI | FdrII | 0.19 (±0.067) | |
| pUKA253 | CarAaI | CarAcI | FdrII | 95 (±4.4) |
| pUKAcarAaII | CarAaII | NDb | ||
| pUKA255 | CarAaII | CarAcII | 0.24 (±0.026) | |
| pUKA257 | CarAaII | FdrI | ND | |
| pUKA261 | CarAaII | CarAcII | FdrI | 40 (±13) |
| pUKA265 | CarAaII | FdxI | FdrI | 0.18 (±0.052) |
| pUKA256 | CarAaII | FdrII | ND | |
| pUKA260 | CarAaII | CarAcII | FdrII | 61 (±1.7) |
a
Conversion ratio (%) = 100 × (peak area for TIC of 2′-aminobiphenyl-2,3-diol)/(peak area for TIC of carbazole + peak area for TIC of 2′-aminobiphenyl-2,3-diol). Values are means ± standard deviations calculated from at least triplicate assays.
b
ND, no product was produced from carbazole.