FIG. 1.

Immunoblotting analysis of rNCL produced by A. oryzae transformants. Culture media of the transformants indicated below were subjected to 15% SDS-PAGE in the presence or absence of 50 mM DTT. (A) The native NCL protein. (B) The strain cotransformed with the separate NAS and NBS expression plasmids (Table 1, pAAsC and pAB rows). (C) The strain cotransformed with the modified separate NAS and NBS expression plasmids (Table 1, pA3GAsC and pA3GB rows). The NCL subunits released from the fusion protein are indicated by arrows a to c. Asterisks indicate the AmyB-NCL fusion protein.