FIG. 2.

Improvement of the level of rNCL production by overexpression of the active form of hacA cDNA. Immunological analysis with anti-NBS antibody was carried out using the culture media of the strain overexpressing the active form of hacA cDNA (+) and the niaD marker-transformed strain (−). The amounts of rNCL produced in the culture medium were calculated by measuring the intensities of the bands and comparing with those of native NBS. The average value of the rNCL production level is shown below the panel.