FIG. 2.

SSCP fingerprint of the V4-V5 regions of bacterial 16S rRNA genes. Lanes: 1, inoculum (cultivated at 7.5% NaCl); 2, 0% NaCl, 42 days; 3, 0% NaCl, 84 days; 4, 7.5% NaCl, 42 days; 5, 7.5% NaCl, 84 days; 6, 15% NaCl, 42 days; 7, 15% NaCl, 84 days; 8, 20% NaCl, 42 days; 9, 20% NaCl, 84 days. The bands marked by arrows were cut from the gel, and the DNA was extracted, reamplified, and sequenced. When direct sequencing failed, the reamplified DNA was cloned, and a representative of each ARDRA pattern was sequenced. The results are listed in Table 3.