TABLE 1.
Quantities of degraded diesel fuel and general microbial parameters of cultures
| Parameter | % NaCl | Valueb at (day):
|
||
|---|---|---|---|---|
| 0 (start)a | 42 | 84 | ||
| Degraded diesel fuel (% of total amt) | 0 | ND | 60 | |
| 7.5 | ND | 65 | ||
| 15 | ND | 47 | ||
| 20 | ND | 9.8 | ||
| Protein content (μg/ml) | 0 | 61.2/62.8 | 70.5/72.5 | |
| 7.5 | 50.1/50.7 | 93.0/93.8 | 96.3/99.9 | |
| 15 | 80.2/83.4 | 86.2/90.0 | ||
| 20 | 31.6/34.4 | 26.2/27.8 | ||
| CFU (ml−1) | 0 | 2.5 × 107 (±0.5 × 107) | 5.7 × 106 (±0.5 × 106) | |
| 7.5 | 4.9 × 107 (±0.5 × 107) | 6.1 × 107 (±0.5 × 107) | 1.7 × 107 (±0.3 × 107) | |
| 15 | 8.6 × 107 (±0.6 × 107) | 4.7 × 107 (±0.4 × 107) | ||
| 20 | 1.3 × 107 (±0.3 × 107) | 4.7 × 106 (±0.4 × 106) | ||
| Total cell count (ml−1) | 0 | 1.8 × 108 (±0.5 × 108) | 8.7 × 107 (±1.6 × 107) | |
| 7.5 | 2.1 × 108 (±0.2 × 108) | 4.2 × 108 (±1.6 × 108) | 1.7 × 108 (±0.1 × 108) | |
| 15 | 5.3 × 108 (±0.6 × 108) | 4.6 × 108 (±0.7 × 108) | ||
| 20 | 1.6 × 108 (±0.3 × 108) | 1.1 × 108 (±0.1 × 108) | ||
| Plating efficiency (CFU/total cell count) | 0 | 0.138 | 0.066 | |
| 7.5 | 0.233 | 0.145 | 0.100 | |
| 15 | 0.162 | 0.102 | ||
| 20 | 0.081 | 0.043 | ||
a
The inoculum was cultivated in the presence of 7.5% NaCl, and thus, no values for other concentrations exist.
b
Maximum values are printed in boldface. Values separated by slashes represent results from duplicate determinations. ND, not done.