Log spore counts (N) of C. botulinum TMW 2.357 spores after combined pressure/temperature treatments with isothermal holding times in THB (pH 5.15). Spore counts are depicted relative to the spore counts of untreated samples (N0). (A) 70°C; (B) 80°C; (C) 90°C; (D) 100°C; (E) 110°C; (F) 120°C. The pressure level was 600 MPa (•), 800 MPa (○), 900 MPa (▪), 1,000 MPa (□), 1,100 MPa (▾), 1,200 MPa (▿), 1,400 MPa (⧫), or 0.1 MPa (◊). Lines dropping below the x axis indicate spore counts below the detection limit [log(N/N0) = −6.5].