TABLE 1.

Oligonucleotide primers used during nested PCRs to amplify P_IB-type ATPases and numbers of isolates which yielded amplicons

PCR	Primerc	Primer sequence	Target genus/genera	Annealing temp (°C)	No. of Pbr isolates that yielded a PCR product
1a	79JC	5′ TGACTGGCGAATCGGTBCCBG 3′	Bacillus, Acinetobacter, Pseudomonas	59	8
	84JC	5′ GGAGCATCGTTAATDCCRTCDCC 3′
	132JC	5′ CTAACTGGCGAATCAGTCCC 3′	Arthrobacter	55	25
	84JC	5′ GGAGCATCGTTAATDCCRTCDCC 3′
2b	81JC	5′ GGATGTCCTTGTGCTYTART 3′	Bacillus, Acinetobacter, Pseudomonas	49	5
	84JC	5′ GGAGCATCGTTAATDCCRTCDCC 3′
	133JC	5′ CCCTCACCTTGTGCYCTGG 3′	Arthrobacter	49	23
	84JC	5′ GGAGCATCGTTAATDCCRTCDCC 3′

^aExpected product size, approximately 1.2 kb.

^bExpected product size, approximately 0.75 kb.

^cSee reference 11 for specific thermocycling parameters.