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. 2006 May;72(5):3504–3514. doi: 10.1128/AEM.72.5.3504-3514.2006

FIG. 1.

FIG. 1.

PCR-SSCP analysis of ISPα gene segments obtained from reference strains. The following strains were analyzed: P. putida F1 (todC1, lane 1); B. xenovorans LB400 (bphA1, lane 2); C. necator H850 (bphA1, lane 3); Pseudomonas sp. strain IC (bphA1, lane 4); R. globerulus P6 (bphA1, lane 5); P. pseudoalcaligenes KF707 (bphA1, lane 6); P. aeruginosa JI104 (bnzA, lane 7); Ralstonia sp. strain PS12 (tecA1, lane 8); Pseudomonas sp. strain Cam-1 (bphA1, lane 9); Pandoraea sp. strain JB1* (bphA1, lane 10); and P. fluorescens IP01 (cumA1, lane 11). Lane 12 shows the PCR-negative control. Molecular weight marker III (M) (250 ng per lane; Roche) was used to normalize the band patterns.