TABLE 2.
Substrate utilization profiles and genotypic characteristics of BTEX isolatesa
| Isolate name | Growth substrateb
|
C23O of subfamily I.2.A | C23O typec | ISPα typed | ISPα/C23O combinationf | ARDRA typeg | ||
|---|---|---|---|---|---|---|---|---|
| Benzene | Toluene | Ethylbenzene | ||||||
| IA1YICDA | − | + | − | + | D | − | PS2 | |
| IA1YICDB | − | + | − | + | D | − | PS2 | |
| 3YC1b | + | + | − | − | − | ND | ||
| 3YC2 | + | + | + | − | Ae | I | PS2• | |
| 3YC4 | + | + | + | − | A | I | PS2• | |
| 3YBTEX2b | + | + | + | − | A | I | PS2 | |
| 1XXyl1 | + | + | + | − | A | I | ND | |
| 1YC1 | + | + | + | + | ND | Ae | PS1• | |
| 1XC2 | + | + | − | + | ND | Ae | PS2 | |
| 1YXyl2 | + | + | + | + | A• | A | II | PS1 |
| 1YXyl3 | + | + | + | + | A• | A | II | PS1 |
| 1XBTEX3 | + | + | + | +• | A• | A | II | PS1 |
| 1XBTEX4 | + | + | + | + | A• | A | II | PS1 |
| 1YXyl1 | + | + | + | + | A2 | Ae | II | PS1 |
| 1YdBTEX2 | + | + | + | + | A2 | A | II | PS1 |
| 1XBTEX1 | + | + | + | + | B | A | III | PS1 |
| 1XB2 | + | + | + | + | B | A | III | PS1 |
| 1XC1 | + | + | + | +• | C• | Ae | IV | PS1 |
| 3YXyl2 | + | + | − | +• | C• | Ae | IV | PS2 |
| 3YC5 | + | + | − | + | C• | Ae | IV | PS2 |
| 3YdBTEX2 | + | + | + | + | C | Ee | V | PS2• |
| 1YB1 | + | − | − | + | A• | C | VI | PS1 |
| 1YB2 | + | − | − | + | A1 | Ce | VI | PS1 |
| 1YB3 | + | − | − | + | A1 | C | VI | PS1 |
| 1YC2 | + | − | − | + | A1 | Ce | VI | PS1 |
| 1YC3 | + | − | − | + | A1 | C | VI | PS1 |
| 1YBTEX1 | + | − | − | + | A1 | C | VI | PS1 |
| 1YBTEX3 | + | − | − | + | A1 | C | VI | PS1 |
| 1YdBTEX3 | + | − | − | + | A1 | C | VI | PS1 |
| 3YdBTEX1b | + | − | − | + | A1 | C | VI | PS2 |
| 3YXyl2b | + | − | − | + | A1 | Ce | VI | PS2 |
| 3YXyl3 | + | − | − | + | A1 | C | VI | PS2 |
| 3YXyl1 | + | + | − | + | A1 | Ce | VI | PS2 |
| 1XB1 | + | − | − | + | A• | C**e | VII | PS3 |
| 3YC3 | + | − | − | − | Be | VIII | AR• | |
| 1XXyl1b | + | − | − | − | De | IX | SP• | |
Data on substrate utilization, presence/type of C23O gene, and ARDRA type were reported previously (37); data indicated by • were obtained in this study by using previously reported procedures (36, 37).
+, growth on substrate vapor; −, no growth on substrate vapor.
C23O types marked by • were identified by AFDRA as reported previously (36), whereas all other C23O types were previously identified by using both AFDRA and DNA sequencing (37). ND, not determined.
ISPα type was determined by sequencing of reamplified SSCP products; −, no PCR product.
ISPα sequence was also determined by sequencing of cloned PCR products amplified with primers bphAf371B and bphAr1153-2.
Roman numerals are used to distinguish between different combinations of C230 and ISPα genotypes observed in a given isolate.
The major portion of isolates from the highly contaminated site 1 was closely related to Pseudomonas veronii CIP 104663 (AF064460, ARDRA profile PS1), whereas isolates from the slightly contaminated site 3 were predominantly related to Pseudomonas mandelii CIP 105273 (AF058286, ARDRA profile PS2). Strain 3YC3 exhibited an ARDRA fingerprint identical to those of previously analyzed Arthrobacter isolates (e.g., AY512644) and was thus considered to be a member of the genus Arthrobacter. Strain 1XXyl1b had previously been identified as a member of the genus Sphingomonas (AY512601) (34).