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Production of recombinant proteins in the biofilm production-deficient strain E. coli BD123. MG1655 and BD123 carrying pEndo (A), pHOPE (B), and both pHOPE and pABC-ACYC (C) were grown for 24 h in 24-well plates. The amounts of lipase and endoxylanase activities in the cytoplasmic space and the supernatant were determined by spectrophotometry (refer to Materials and Methods).
