Figure 3.

o-Dbl and PLCγ1 partially restore degranulation in B6A4C1 cells. B6A4C1 cells were infected with either 60 pfu/cell of empty vector, Cdc42^V12, or o-Dbl, or 20 pfu/cell of empty vector, Cdc42^V12, or PLCγ1 for 6 h at 37°C. Degranulation assays were performed as described in Fig. 1 and Materials and Methods. Error bars represent the standard deviation of at least three independent experiments. B6A4C1 cells infected with 60 pfu/cell of o-Dbl (Inset, lane 2) or 20 pfu/cell of PLCγ1 (Inset, lane 4) for 6 h at 37°C, or left uninfected (Inset, lanes 1 and 3) were lysed, proteins were resolved by SDS/PAGE, and the virus-expressed proteins were detected by Western blotting by using anti-Dbl or anti-PLCγ1 antibodies.