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. 2006 May;72(5):3103–3110. doi: 10.1128/AEM.72.5.3103-3110.2006

FIG. 1.

FIG. 1.

PCR-amplified product of 1,025 bp obtained with the 16S rRNA-specific primer pair GB-F and GBN2-R. One-kilobase plus DNA ladder: lanes 1, 8, 16, and 23. No-DNA control, lane 30. PCR amplification in diazotrophic Burkholderia species (except B. vietnamiensis) and closely related organisms; B. unamae: lane 2, MTl-641T, and lane 3, SCTx-181 isolated from rhizosphere of sugarcane (Mexico); B. tropica: lane 6, Ppe8T, and lane 7, SCTx-269 isolated from surface-sterilized roots of sugarcane; type NAR isolates: lanes 11 and 12, SRMrh-20 and SRCL-318 isolated from rhizosphere of maize and surface-sterilized leaves of sugarcane, respectively; lane 15, B. graminis CRD1MT; lane 18, B. caribensis MWAPT; lane 19, B. caledonica LMG 19076T; line 22, B. tuberum STM-678T; line 24, B. phenazinium LMG 2247T; lane 26, B. sacchari IPT101T; lane 27, B. phymatum STM815T; lane 29, B. xenovorans LB400T. PCR-negative amplification in B. cepacia complex species and closely related species: lane 4, B. vietnamiensis TVV75T; lane 5, B. multivorans LMG 13010T; lane 9, B. gladioli ATCC10248T; lane 10, B. cepacia ATCC25416T; lane 13, B. cenocepacia J2315T; lane 14, B. glumae LMG 2196T; lane 17, B. pyrrocinia ATCC15958T; lane 20, B. ambifaria LMG 11351; lane 21, B. caryophylli LMG 2155T; lane 25, Ralstonia pickettii LMG 5942T; lane 28, Ralstonia solanacearum LMG 2299T. Other Burkholderia species tested (data not shown): PCR-positive amplification, B. hospita LMG20598T, B. terricola LMG20594T, B. phytofirmans PsJNT, and B. fungorum LMG 16225T; PCR-negative amplification, B. stabilis LMG 6997.