Protein electrophoregrams of representative acetylene-reducing isolates recovered in the present study and type strains of known diazotrophic Burkholderia species. B. tropica: lane 1, MTl-632 isolated from rhizosphere of maize, and lane 2, Ppe8T; B. unamae: lane 3, MTl-641T; lane 4, SRCrh-274 isolated from rhizosphere of sugarcane (Brazil); type NAR isolates: lane 5, SRMrh-20, and lane 6, SRCL-318; lane 7, B. cepacia ATCC25416T.