TABLE 2.

Groups of Lactobacillus species, oligonucleotide primer sequences and locations, and DGGE conditions

Lactobacillus group and organism	Multiplex PCR primersa	DGGE primersb	DGGE conditionsc
1 L. paraplantarum L. pentosus L. plantarum	Gru1′"F, Gru2"F, Gru3F, Gru4F, LrosF, LrosF, MulISRR	60g1F (5′-GGCATTCGTCGTGGGATTGAAGAAGC-3′ [40-65, AY424357])/GC-60g1R (5′-CCATGTATTGTGATAAGTAGCCGCGG-3′ [282-307, AY424357 reversed])	9% (wt/vol) polyacrylamide gel in 1× TAE, denaturing gradients (FU) 41-46%
2 L. alimentarius L. farciminis L. mindensis L. paralimentarius	Gru1′"F, Gru2"F, Gru3F, Gru4F, LrosF, LrosF, MulISRR	Gru2"F/GC-MulISRR	9% (wt/vol) polyacrylamide gel in 1× TAE, denaturing gradients (FU) 41-46%
3 L. brevis L. fructivorans L. hilgardii L. sanfranciscensis	Gru1′"F, Gru2"F, Gru3F, Gru4F, LrosF, LrosF, MulISRR	Nest3F (5′-GGGGTTGTAGGACTGAACATTTG-3′ [512-534, AJ616221])/GC-MulISRR	9% (wt/vol) polyacrylamide gel in 1× TAE, denaturing gradients (FU) 41-46%
4 L. fermentum L. frumenti L. panis L. pontis	Gru1′"F, Gru2"F, Gru3F, Gru4F, LrosF, LrosF, MulISRR	Nest4F (5′-CGATTCCCTGAGTAGCGGC-3′ [438-456, AJ616012])/GC-MulISRR	6% (wt/vol) polyacrylamide gel in 1× TAE, denaturing gradients (FU) 53-58%

^aThe primers written in bold represent primers specific for the group indicated in that row. See reference 15 for primer sequences.

^bPrimer sequences are given in parentheses, and nucleotide positions and GenBank accession numbers are given in brackets.

^cTAE, 40 mM Tris, 20 mM acetic acid, and 1 mM EDTA (pH 8.0); FU, 100% denaturant contained 40% (vol/vol) formamide and 7 M urea.