TABLE 4.
Influence of pH on SCFA concentrations and relative proportions of each strain in monocultures of B. adolescentis L2-32 and Roseburia sp. strain A2-183 and their cocultures when incubated in YCFA medium containing 0.2% potato starch or fructo-oligosaccharides
| SCFA | Concn change (mM) with the following substrate, pH, and inoculuma
|
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Starch
|
FOS
|
|||||||||||||||
| 5.7
|
6.5
|
5.7
|
6.5
|
|||||||||||||
| B | R | R + B | B | R | R + B | B | R | R + B | B | R | R + B | |||||
| Acetate | 15.2 ± 2.2 | −0.8 ± 0.6 | 0.02 ± 3.2 | 18.0 ± 1.4 | 2.4 ± 1.4 | 5.3 ± 1.2 | 6.3 ± 2.1 | −1.3 ± 0.7 | 7.3 ± 1.9 | 12.1 ± 2.4 | −1.1 ± 1.4 | 11.5 ± 3.1 | ||||
| Butyrate | 2.5 ± 0.1 | 8.1 ± 1.8 | 2.6 ± 0.2 | 6.1 ± 0.9 | 1.5 ± 0.1 | 5.1 ± 0.4 | 1.5 ± 0.1 | 9.9 ± 2.1 | ||||||||
| Lactate | 6.5 ± 0.6 | 1.9 ± 0.7 | 3.2 ± 0.2 | 0.4 ± 0.1 | 1.4 ± 0.1 | 1.1 ± 0.1 | 1.3 ± 0.2 | 0.7 ± 0.1 | 1.8 ± 0.7 | |||||||
| R/Bb | 0.60 | 2.07 | 0.25 | 1.34 | 0.31 | 0.41 | 0.41 | 1.69 | ||||||||
a
Values are means of three replicates ± standard deviations. B, B. adolescentis L2-32; R, Roseburia sp. strain A2-183; R + B, coculture.
b
R/B, ratio of abundances of Roseburia sp. strain A2-183 and B. adolescentis L2-32 in pellets obtained from the combination of equal volumes of the cocultures or the monocultures of both strains.