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. Author manuscript; available in PMC: 2006 May 30.
Published in final edited form as: J Biol Chem. 2005 Dec 14;281(6):3382–3388. doi: 10.1074/jbc.M511295200

Fig. 2. Endogenous GPx-1 protein is increased by G418 treatment.

Fig. 2

COS7 cells were cultured in selenium-deficient media (no Se) or selenium-replete media (Se, plus 10 ng/ml sodium selenite). After 7 days, treatments were continued for 3 additional days with the addition of G418 (200 μg/ml). (A) Western blot with a mouse anti-human GPx-1 antibody. (B) GPx-1 enzyme activity was measured in extracts; upper plot shows mU/mg ± standard deviations; lower plot shows GPX-1 activity normalized for band density, relative to the Se condition which was arbitrarily set to 1.