Fig. 3.

Production of chimeric animals by heterozygous occludin knockout mGS cells. (A) Appearance of the mGS cells. (B) Southern blot analysis of the mGS cell lines. (C) Section of a teratoma from the mGS cells after gene targeting. Note the presence of tissues from three germ layers, which include muscle (m), neural (n), and epithelial (e) tissues. (D–H) Macroscopic appearance of the brain (D), heart (E), gut (F), kidney (G), and ovary (H), showing fluorescence under UV light. Arrows indicate oocyte-like cells in the ovary. (Scale bars: A and C, 100 μm; D–H, 1 mm.)