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. 2006 May 5;103(21):8018–8023. doi: 10.1073/pnas.0601139103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 4. — Effects of genetic manipulation on karyotype and imprint patterns in GS and mGS cells. (A) Karyotype analysis of the heterozygous knockout GS and mGS cells before and after genetic selection. At least 20 cells were counted. (B) Combined bisulfite restriction analysis (COBRA) of imprinted genes. DNA was isolated from heterozygous knockout GS (clones 7 and 101) and mGS (clone 118) cells. Heterozygous F₁ offspring were produced from GS clone 101. PCR products from differentially methylated regions were digested with enzymes with a recognition sequence containing CpG in the original unconverted DNA. The percentage methylation is indicated below the gel. Enzymes used to cleave each locus are indicated in parentheses. U, uncleaved; C, cleaved. F, female; M, male.