Table 2.
Germ-line transmission of mutagenized GS cell lines by germ cell transplantation
| Type of experiments | Clone | LacZ stain | Days from transfection to transplantation* | Days to first transgenic† | No. (%) of fertile out of total recipients | No. of litters analyzed | No. (%) of transgenic out of total offspring‡ |
|---|---|---|---|---|---|---|---|
| Gene trap | 6 | + | 64 | 109 | 2/7 (28.6) | 2 | 9/12 (75.0) |
| 7 | + | 64 | 82 | 1/7 (14.3) | 5 | 10/21 (47.6) | |
| 13 | + | 52 | 77 | 3/5 (60.0) | 6 | 38/46 (82.6) | |
| 14 | − | 78 | 85 | 3/8 (37.5) | 3 | 6/15 (40.0) | |
| 27 | + | 52 | 80 | 2/3 (66.7) | 5 | 26/35 (74.3) | |
| 30 | − | 57 | 89 | 2/9 (22.2) | 5 | 15/24 (62.5) | |
| 39 | − | 57 | 89 | 2/4 (50.0) | 4 | 12/27 (44.4) | |
| Gene targeting | 101 | NA | 109 | 109 (69)§ | 3/5 (60.0) | 2 | 10/14 (71.4) |
Results at 4 months after transplantation. NA, not applicable.
*Time in days from transfection of GS cells to transplantation into infertile recipients.
†Time in days from transplantation of donor cells to birth of first transgenic progeny.
‡Numerator is the number of transgenic progeny; denominator is the total number of progeny from all recipient mice.
§In parentheses is the number of days for first offspring by in vitro microinsemination.