Table 1.
Retrotransposition in primary human fibroblasts and hepatocytes
A/RT virus amount used for infection, moi | Hepatocyte |
Fibroblast |
||||
---|---|---|---|---|---|---|
Exp. 1 |
Exp. 2 |
|||||
β-gal | EGFP | β-gal | EGFP | β-gal | EGFP | |
0 | 0 | 0.1 | 0 | 0.1 | 0 | 0.1 |
0.1 | 10 | 0.3 | 20 | 0.1 | 10 | 0.1 |
1 | 40 | 0.5 | 70 | 0.5 | 70 | 0.9 |
10 | 90 | 2.7 | 90 | 1.5 | 90 | 1.8 |
Cells (2 × 105) were infected with various amounts of A/RT virus and assayed for EGFP and β-gal expression 7 days after infection.