Table 1.
Retrotransposition in primary human fibroblasts and hepatocytes
| A/RT virus amount used for infection, moi | Hepatocyte |
Fibroblast |
||||
|---|---|---|---|---|---|---|
| Exp. 1 |
Exp. 2 |
|||||
| β-gal | EGFP | β-gal | EGFP | β-gal | EGFP | |
| 0 | 0 | 0.1 | 0 | 0.1 | 0 | 0.1 |
| 0.1 | 10 | 0.3 | 20 | 0.1 | 10 | 0.1 |
| 1 | 40 | 0.5 | 70 | 0.5 | 70 | 0.9 |
| 10 | 90 | 2.7 | 90 | 1.5 | 90 | 1.8 |
Cells (2 × 105) were infected with various amounts of A/RT virus and assayed for EGFP and β-gal expression 7 days after infection.