Fig. 6.

Chromatin remodeling during meiosis in hsk1-89 cells. Chromatin remodeling was examined in the ade6-M26 region during meiosis induced in diploid cells D20 (M26 hsk1⁺/M26 hsk1⁺, Left) and D21 (M26 hsk1-89/M26 hsk1-89, Right). The arrowheads indicate the bands derived from the chromatin remodeling observed in the WT cells. Micrococcal nuclease was titrated (0, 5, 10, or 20 units per ml for hsk1⁺/hsk1⁺ and 0, 10, or 20 units per ml for hsk1-89/hsk1-89) at each time point, as indicated by the filled triangles. The open arrow indicates the coding region and direction of the ade6 gene. The positions of the XhoI site, ATG initiation codon, and M26 DSB sites are also indicated. N, MNase digestion of naked DNA.