Fig. 4.

Gα12 protein levels are up-regulated in carcinoma in situ and in invasive adenocarcinoma of the breast. Sections of formalin-fixed paraffin-embedded breast tissue were stained for Gα12 with anti-G12 anti-sera (Santa Cruz Biotechnology) as described in Methods and Materials. Original images were taken with a ×40 objective. (A and B) Benign breast tissue. (C) Ductal carcinoma in situ of the breast. (D) Lobular carcinoma in situ of the breast. (E) Section containing benign breast epithelium, carcinoma in situ, and invasive ductal carcinoma of the breast. (F) Section containing both benign breast epithelium and invasive lobular carcinoma of the breast. (E and F) Arrowheads indicate cancer; arrows indicate benign epithelium.