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The catalytic properties of TodS. (A Upper) Autophosphorylation in the presence and absence of toluene. Experiments were carried out in parallel, and the graph (A Lower) shows the densitometric analysis of the gels. (B) Dephosphorylation kinetics. (C) Transphosphorylation to TodT. Conditions for these assays are described in Materials and Methods.
