. 2006 May 15;103(21):8191–8196. doi: 10.1073/pnas.0602902103

Table 1.

Bacterial strains and plasmids used in this study

Strains/Plasmids	Relevant characteristics	Refs.
E. coli DH5αF′	F′/hsdR17, recA1, gyrA	28
E. coli BL21 (DE3)	F⁻, ompI, hsdS_B (r^−_B m^−_B)	28
P. putida DOT-T1E	Tol⁺, wild type	29
P. putida DOT-T1E todST	DOT-T1E, todST::Km, Tol⁻	30
P. putida DOT-T1E ΔtodT	DOT-T1E, todT::Km, Tol⁻	This work
P. putida KT2440	Tol⁻, Cm^R	31
P. putida KT2440-IHF3	KT2440, ihfA::Km	31
pBSaphA	Ap^R, aphA3 cassette from pU18K	32
pET-28b⁺	Km^R, protein expression vector	28
pET11c-ihfABhis	Ap^R, ihfA and His-tagged ihfB genes	33
pMIR77	Tc^R, P_todX::′lacZ	30
pJLC77	Tc^R, mutant P_todX::′lacZ	This work
pMIR66	Gm^R, containing the todST genes	30
pJLC1	pMIR66, todS::aphA3	This work
pJLC2	pMIR66, todT::aphA3	This work
pJLTodT	pET28b containing todT gene	This work
pTodS	pET28b containing todS gene	This work
pCTodS	pET28⁺: todS; encoding CTodS	This work
pNTodS	pET28⁺: todS; encoding NTodS	This work
pREIIα	Ap^R, ropA	19, 20
pREIIα-Δ235	Ap^R, RpoA Δ235 residues	19, 20
pREIIα-289A	Ap^R, codifies RpoAL289A	19, 20
pREIIα-291A	Ap^R, codifies RpoAK291A	19, 20

Ap^R, Cm^R, Gm^R, Km^R, Rif^R, Tc^R indicate resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, rifampicin, and tetracycline, respectively. Tol⁺ and Tol⁻ indicate either that the strain grows or fails to grow on toluene, respectively.