Figure 3.

G2/M delay in top2-B44 depends on spindle checkpoint proteins, but not the Cdc28 kinase Swe1. Cell cycle analysis of double mutants of top2∷top2-B44 combined with a swe1-null or spindle assembly checkpoint mutants, performed as described in Figure 1 after G1 synchrony. In the case of the GAL1-MIH1 and top2∷top2-B44 GAL1-MIH1 strains, the cells were synchronized in G1in medium containing raffinose, then released into the cell cycle in the presence of galactose to induce overexpression of MIH1. For each time point, at least 200 cells were scored. The averages of several counts are plotted and the error bars show standard deviations. Derived from these data, approximate lengths of the G2/M period are listed for these stains in Supplementary Table 1.