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ATP release from human erythrocytes. ATP was measured with a luciferase assay in the supernatant after centrifugation. Data are normalized to basal release in unstimulated erythrocytes. (A) Release was increased by depolarization with potassium as well as by hypotonic stress with 1:1-diluted Krebs solution. (B) Inhibition of osmotically induced ATP release by 100 μM carbenoxolone. Means ± SEM are plotted (n = 5).
