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. 2006 May 3;103(20):7676–7681. doi: 10.1073/pnas.0602595103

Fig. 2.

Fig. 2.

Appearance of YFP-tagged markers at the new Golgi. (A–D) Fluorescence imaging of fixed cells expressing the indicated constructs (in green) labeled with antibodies to GRASP (red). Panels are arranged from left to right, as the cells progress through the cycle. Cells at left have a single (old) Golgi, cells in the middle have a growing new Golgi, and cells at right have more additional Golgi that appear just before cytokinesis. Arrows indicate first appearance of the new Golgi, and Insets show the separated green and red channels. Note that GntB colocalizes with GRASP at all stages of the cell cycle. In contrast, YFP-ε-COP, ss-YFP, and YFP-GRIP70 are not present at the earliest stage defined by the appearance of GRASP. Furthermore, ss-YFP is sometimes found immediately adjacent to the new Golgi at this early stage (arrowhead). (Scale bar: 5 μm.) (E) Quantitation of the total YFP fluorescence at the old Golgi (early in the cell cycle) in cells expressing the indicated constructs tagged with YFP. Results were expressed as the mean ± SEM (n = 6).