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7SK siRNA distributes throughout CHO cells 24 h after transfection. (a–c) 7SK siRNA antisense strand (red) is seen in the cytoplasm and the nucleus, whereas sense strand (green) gets eliminated. (d) Colocalization shows antisense strand is retained in the nucleoli (red spots) and confirms integrity of the cell nuclei (blue). (Scale bar, 20 μm.) (e) A single cell. (Scale bar, 10 μm.) (f) Intensity profiles along the selected line.
