Fig. 2.

LC-inclusion analysis in LPS-stimulated splenocytes (A) and Peyer’s patch B cells (B). (A) Flow cytometric analysis of LPS-stimulated splenocytes in WT and κCκD mice. Splenocytes stimulated with LPS for 3 days were stained with anti-κ and anti-λ antibodies. (B) For Peyer’s patch B cells, triple-staining with antibodies specific for IgA, κ LC, and λ LC was performed (κ and λ LC expression was checked on IgA⁺-gated cells). Numbers indicate the percentages of cells in one representative of three independent experiments.