Fig. 3.

Detection of human DNA in MIG-transplant goat. (A Top) FACS of samples from perfused liver to enrich for the GFP population. The resulting cell pool was reanalyzed by FACS to assess enrichment, producing the cytogram of cell counts vs. fluorescence. (A Middle and Bottom) Individual cells from the sorted pool were observed under light (Middle) and fluorescence (Bottom) microscopy and compared to normal goat liver cells. (B) FACS to measure DNA content discriminated goat and human cells by total chromosome number. DNA content is shown for perfused human liver (a), normal goat liver (b), MIG goat liver (c), and sorted GFP⁺ cells from perfused MIG goat liver (d). H, human, 2n = 46 chromosomes; G, goat, 2n = 60 chromosomes.