Fig. 1.

SOCS3 is induced by LIFR signaling by oligodendrocytes in vitro and in vivo in the context of cuprizone-mediated demyelination. (a) Northern blot analysis of SOCS3 expression in primary rat oligodendrocytes treated with LIF. (b) Western blot analysis of SOCS3 expression in CG-4-derived oligodendrocytes treated with LIF. (c) Immunostaining for CC1 and SOCS3 in the white matter of an unchallenged mouse and a mouse given 0.2% cuprizone for 10 days. Merged images include DAPI nuclear stain (blue). (Scale bar, 25 μm.) (d) RT-PCR analysis of SOCS3, myelin basic protein (MBP), LIF, and CNTF in samples isolated from the corpus callosum by laser capture microdissection (LCM). Mice were either unchallenged or given cuprizone for 10 days, and they were wild type or mutant for the LIF and CNTF genes as indicated. Template loading uniformity between samples was assessed by amplification of GAPDH.