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. 2006 May 8;103(20):7859–7864. doi: 10.1073/pnas.0602574103

Fig. 3.

Fig. 3.

Generation and confirmation of conditional knockout of SOCS3 within oligodendrocytes. (a) Northern blot analysis of Cre expression in MBP–Cre transgenic mice. (b) BamHI digestion and Southern blot analysis of DNA from the CNS and immune organs of a SOCS3FL/FL and a SOCS3ΔMBP/ΔMBP mouse distinguish between the loxP-flanked (SOCS3FL, 9 kb) and excised allele (SOCS3DEL, 4.9 kb). The image shown is representative of three mice examined for each genotype. (c) RT-PCR analysis of genomic DNA isolated from the LCM samples [cortex (Cx) or white matter (WM)] of a SOCS3ΔMBP/ΔMBP mouse, showing white-matter predominance of Cre and MBP gene expression. (d) PCR analysis of genomic DNA isolated from the LCM samples (cortex or white matter) of a SOCS3FL/FL and a SOCS3ΔMBP/ΔMBP mouse, distinguishing between the SOCS3 loxP-flanked (FL) and excised (DEL) alleles.