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Immunoblot analysis of Sig1 under −S conditions. Total proteins (7.5 μg) from times 0, 4, 8, and 12 h after starvation from WT and sac3 cells (A) and WT cells treated with DMAP or DMSO (B) were separated in SDS-polyacrylamide gels and transferred to nitrocellulose. Blots were probed first with anti-Sig1 and then with anti-α-tubulin. Densitometric quantification of Sig1 and tubulin levels is shown. The relative values (from time 0) represent the mean ± SD of two independent experiments.
