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. 2006 Jun;80(12):5822–5832. doi: 10.1128/JVI.02707-05

FIG. 3.

FIG. 3.

gM interferes with UL49.5-mediated inactivation of TAP. (A) UL49.5 inhibition of TAP-dependent peptide transport is not observed in MJS UL49.5/gM cells. The translocation of fluorescent peptides in MJS UL49.5 or MJS UL49.5/gM cells is represented as a percentage of the translocation in control (c) MJS cells. FL, fluorescein. (B) UL49.5/gM coexpression results in the stabilization of TAP1 and TAP2 protein levels. Steady-state levels of TAP1, TAP2, and beta-actin were evaluated by immunoblotting in control (c) MJS, MJS UL49.5, MJS gM, or MJS UL49.5/gM cells using specific antibodies; *, unidentified background protein. (C) Downregulation of MHC class I surface expression by UL49.5 in the absence (upper panel, boldface line, no. 2) or in the presence of gM (lower panel, boldface line, no. 3). Thin line, MHC I in MJS cells (no. 1); dashed line, goat anti-mouse phycoerythrin control (c). (D) Immunoblot analysis of UL49.5 expression in MJS cells expressing UL49.5 or coexpressing UL49.5 and gM. Uninfected and BHV-1-infected MJS cells were included as controls.