FIG. 4.

UL49.5 and gM are expressed with different kinetics during BHV-1 infection. MJS cells were infected with BHV-1 in the absence or presence of phosphonoacetic acid (−PAA and +PAA, respectively), collected at the indicated time points postinfection (hpi), and metabolically labeled for 35 min. (A) UL49.5 (left panel) or gM (right panel) was immunoprecipitated from cell lysates with anti-UL49.5 and anti-gM antibodies, respectively. The fully glycosylated gM (solid circle), the high-mannose gM (open circle), and the gM precursor (dashed circle) are indicated. (B) The early glycoprotein B or the late virion host shutoff (vhs) protein were immunoprecipitated from the cell lysates as controls. (C) The experiment is the same as that described for panel A, performed in MDBK cells. (D) The early gB, the late gC, and the late vhs protein immunoprecipitated from the MDBK cell lysates. Size markers are in kilodaltons.