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. 2006 Jun;80(12):5822–5832. doi: 10.1128/JVI.02707-05

FIG. 5.

FIG. 5.

BHV-1 inhibits ATP-dependent peptide transport by TAP at late stages of infection. MJS cells were infected with wild-type (wt) BHV-1 or a virion host shutoff deletion (vhs−) mutant for 12 h. (A) TAP-dependent peptide transport was assessed in the presence or absence of ATP using a fluorescein (FL)-tagged peptide. Uninfected MJS cells were used as a control. FL-peptide translocation in infected cells is represented as a percentage of the translocation in uninfected cells (set as 100%). (B) gM and UL49.5 detected in the BHV-1-infected cells used in the TAP transport assays shown in panel A. The cell lysates were separated by SDS-PAGE and analyzed by immunoblotting using antibodies against gM (upper panel) or UL49.5 (lower panel). Size markers are in kilodaltons. (C) The viability of the BHV-1-infected cells was evaluated at 12 hpi by 7-AAD staining and flow cytometry. FSC, forward scatter.