TABLE 2.
Reciprocal frequency of MHV-68 infection in GC B cells in vav1- and vav2-deficient micea
| Days postinfection | Mice | Reciprocal frequencyb of viral DNA-positive cells | % Purityc | % Cellsd | Total no. of cellse | No. of viral DNA-positive cellsf |
|---|---|---|---|---|---|---|
| 14 | wt | 158 (96-434) | 99.5 | 2.4 | 4.8 × 106 | 30,380 |
| vav1−/− | 93 (60-208) | 98.4 | 4.9 | 9.8 × 106 | 105,376 | |
| vav2−/− | 46 (28-125) | 99.1 | 4.0 | 8 × 106 | 173,913 | |
| 50 | wt | 1,093 (697-2,532) | 95.8 | 2.1 | 4.2 × 106 | 3,843 |
| vav1−/− | 10 (7-26) | 97.0 | 2.4 | 4.8 × 106 | 480,000 | |
| vav2−/− | 5 (3-11) | 95.9 | 2.1 | 4.2 × 106 | 840,000 |
a
Data were obtained from pools of at least five spleens. wt, wild type.
b
Frequencies were calculated by limiting-dilution analysis with 95% confidence intervals (numbers in parentheses).
c
The purity of sorted cells was determined by fluorescence-activated cell sorting (FACS) analysis.
d
The percentage of each population of total spleen was determined by FACS analysis.
e
The total number of cells was estimated from the percentage of the total spleen, based on an estimate of 2 × 108 cells/spleen.
f
The number of latently infected cells was based on the frequency of latency within each cell type and the estimated total number of cells.