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Effect of NH4Cl on RA59/MHV-2SS757R replication in L2 cells. Cells were either pretreated from 1 h before virus infection to 1 h p.i. or treated from 1 to 3 h p.i. with 40 mM NH4Cl. After treatment, medium containing NH4Cl was removed from cells and replaced with fresh growth medium. Culture supernatants were collected at 16 h p.i., and viral titers were determined by plaque assay. Error bars represent the standard deviations from three replicates.
