TABLE 3.
Mapping of anti-S MAbs targeting linear epitopes by peptide-based ELISA
MAb | OD450 of S peptide from aaa:
|
||||||
---|---|---|---|---|---|---|---|
236-253 | 528-545 | 544-558 | 549-566 | 807-823 | 814-831 | 1125-1141 | |
Anti-S1-NT | |||||||
114E3 | 0.43 | 0.01 | 0.03 | 0.01 | 0.02 | 0.02 | 0.03 |
115H2 | 1.25 | 0.02 | 0.03 | 0.02 | 0.03 | 0 | 0 |
116F8 | 1.26 | 0.03 | 0.01 | 0.02 | 0.03 | 0.01 | 0.02 |
Anti-S1-CT | |||||||
114G5 | 0 | 0.41 | 0.01 | 0.02 | 0 | −0.01 | 0.01 |
101F10 | −0.01 | 0.01 | 2.71 | 2.94 | 0.02 | 0 | −0.01 |
103F2 | 0.04 | 0.03 | 2.65 | 2.86 | 0.03 | 0.03 | 0.02 |
104D4 | 0.01 | 0.03 | 2.71 | 2.79 | 0.02 | 0.03 | 0.01 |
111A7 | 0.02 | 0.03 | 2.89 | 3.03 | 0.01 | 0.03 | 0.02 |
121B8 | 0.02 | 0.03 | 2.51 | 2.89 | 0.02 | 0 | −0.01 |
Anti-S2-NT 102D7 | 0.02 | 0.03 | 0 | 0.01 | 3.16 | 2.87 | 0.03 |
Anti-S2-CT 119F6 | 0.02 | 0.03 | 0.01 | 0.02 | 0.03 | 0.01 | 0.59 |
ELISA plates were coated with each of the peptides at 5 μg/ml, and MAbs were tested at 10 μg/ml. An optical density at 450 nm (OD450) of >0.2 was considered a positive reaction and is highlighted in boldface type.