FIG. 8.

Treatment with sodium orthovanadate reverses the blocking effect of IFN-stimulated Stat1 and Tyk2 phosphorylation in JEV- and NS5-SIN-infected cells. Vero cells pretreated with or without sodium orthovanadate (25 μM) for 16 h were mock infected or infected with wt-SIN, NS5-SIN, or JEV (MOI = 10) in the presence or absence of sodium orthovanadate (25 μM) for 6 h as indicated above the lanes. The cells were then stimulated with IFN-αA/D (1,000 U/ml) for 30 min (or 15 min for Tyk2) or left unstimulated before the cell lysates were harvested for Western blot analyses using anti-phospho-Stat1 (Tyr701), anti-Stat1, anti-phospho-Tyk2 (Tyr1054/1055), anti-Tyk2, anti-Flag, and anti-JEV NS3 antibodies as shown on the right sides of the gels.