TABLE 2.

Level of replication of rHMPV and ΔSH/G-F_WT and ΔSH/G-F_AMPV-A derivatives in the upper and lower respiratory tracts of AGMs

Virusa	Nasopharyngeal swab			Tracheal lavage
	Duration of shedding (days ± SE)	Mean peak titerb (log10 PFU/ml ± SE) and statistical groupingc	Reduction of mean peak titerd (log10 PFU/ml)	Duration of shedding (days ± SE)	Mean peak titerb (log10 PFU/ml ± SE) and statistical groupingc	Reduction of mean peak titerd (log10 PFU/ml)
rHMPV	9.3 ± 0.6	3.8 ± 0.1, A		8.5 ± 1.0	5.4 ± 0.2, A
FWT	3.5 ± 0.9	2.1 ± 0.3, B	1.7	4.0 ± 1.0	3.1 ± 0.1, B	2.3
FAMPV-A	4.3 ± 1.2	1.3 ± 0.3, B	2.5	3.0 ± 0.0	2.6 ± 0.1, B	2.8

^aAGMs in groups of four were inoculated on day 0 simultaneously by the intranasal and intratracheal routes by using a 1-ml inoculum per site containing 6.0 log₁₀ PFU of F_WT or F_AMPV-A. Two animals were inoculated with 6.0 log₁₀ PFU of rHMPV in parallel; results from 10 additional animals that were inoculated with rHMPV under identical conditions in previous experiments were included.

^bThe level of virus replication is expressed as the geometric mean of the peak virus titers (log₁₀ PFU/milliliter ± standard errors) for the animals in each group irrespective of sampling day. The lower limit of detection was 0.7 log₁₀ PFU/ml. A value of 0.7 log₁₀ PFU/ml was assigned to samples with no detectable virus.

^cMean peak virus titers were assigned to statistically similar groups by the Tukey-Kramer post hoc test. Values within a column that share a common letter are not significantly different; values with different letters are significantly different (P < 0.05).

^dReduction of mean peak titer compared to that of rHMPV.