TABLE 3.
Inhibitory activity of MAbs against HIV-1BaL, Bx08, and TV1 replication in macrophages
| Protein | MAb name | Epitope (sequence or domain) | IC90 (μg/ml)a
|
|||||
|---|---|---|---|---|---|---|---|---|
| PBMC
|
iDC | Macrophages
|
||||||
| BaL | Bx08 | BaL | BaL | Bx08 | TV1 | |||
| gp120 | 221 | gp160/gp120 (aa 482-495) | >50 | >100 | ND | 20b | 50b | >100 |
| V3 loop | IIIB-V3-13 | V3 (IRIQRGPGRAFTIGC) | >50 | >100 | ND | 3 | ND | ND |
| 257-D IV | V3 (KRIHI sequence) | >9.4 | ND | 10c | 0.05e | 0.3d | >9 | |
| 268-D IV | V3 (HIGPGR sequence) | >14 | >14 | 15b | 1d | 0.5d | >15 | |
| MN215 | V3 (KS/GIHIGPGKAFYTTGEI) | >125 | >125 | >50 | 10c | 40b | 20b | |
| 391-D | V3 | 20b | 50b | ND | 1d | 0.8d | 20b | |
| F425B4a1 | V3 | >33 | ND | ND | 0.4d | ND | ND | |
| F425B4e8 | Base of V3 loop | 25b | 100b | 4c | 1d | 5c | >30 | |
| gp41 | 240-D | gp41 (aa 579-604) (I)f | >35 | >35 | 20b | 1.8d | 1.9d | 0.5d |
| 246-D | gp41 (aa 579-604) (I) | >100 | >100 | 45b | 0.8d | 0.5d | 0.06e | |
| 50-69 | gp41 (aa 579-613 conformational) | >100 | >100 | >50 | 0.4d | 0.8d | 0.6d | |
| F240 | gp41 (aa 592-606) | >100 | >100 | 50b | 0.5d | 0.6d | 15b | |
| 1577 | gp41 (aa 731-752) | >100 | >100 | >100 | 25b | 100b | >100 | |
a
Values correspond to the ID90 (μg/ml) determined when PHA-stimulated PBMC, macrophages, or iDC were used as target cells. ND, not done.
b
Range, 11 to 100 μg/ml.
c
Range, 2 to 10 μg/ml.
d
Range, 0.3 to 1.9 μg/ml.
e
Range, 0.05 to 0.2 μg/ml.
f
Immunodominant domain of gp 41 (cluster I).