Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Jun;80(12):5841–5853. doi: 10.1128/JVI.02698-05

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, American Society for Microbiology

PMC Copyright notice

FIG. 1. — Baits for aptamer screens. (A) Diagrams of the TGMV AL1 coding regions (TAL1_1-352 and TAL1_1-130) cloned downstream of the LexA DBD are shown. Motifs I, II, and III, which are associated with rolling-circle replication initiator proteins, are marked by the black boxes, and their consensuses are shown (39, 43). The oval corresponds to a conserved helix-loop-helix motif, and the gray box is the ATP binding motif. (B) Baits were tested for oligomerization activity using the positive (AD:TAL1_1-352) and the negative (AD:Jun) prey controls. The yeast transformants are TAL1_1-352 plus AD:TAL1_1-352 (1), TAL1_1-352 plus AD:Jun (2), TAL1_1-130 plus AD:TAL1_1-352 (3), TAL1_1-130 plus AD:Jun (4), GUS plus AD:TAL1_1-352 (5), GUS plus AD:Jun (6), CaAL1_1-349 plus AD:TAL1_1-352 (7), and CaAL1_1-349 plus AD:Jun (8). Interaction was monitored by growth on Gal−HWUL medium. Growth on Glu−HWU medium controlled for plasmid selection, whereas no growth on Glu−HWUL verified that the interaction is dependent on the induction of prey plasmid expression.