TABLE 4.
Aptamers isolated in screens with TAL11-130
| Aptamer | Peptide sequence | Yeast plasmida | Expression cassetteb | Aptamer | Peptide sequence | Yeast plasmida | Expression cassetteb | |
|---|---|---|---|---|---|---|---|---|
| A-3 | GFRAPGLSPTRPSCLICSTL | B1162-3 | pNSB1228 | A-114 | VRSHRRYQRNWEPVVSWFSS | B1162-114 | pNSB1279 | |
| A-5 | NECLICHMLGIREFGLSA | B1162-5 | pNSB1229 | A-115 | WCGPQVSARCK | B1162-115 | pNSB1253 | |
| A-6 | GTLWRRCASSWAFPPDCPSA | B1162-6 | pNSB1230 | A-116 | SCDEAFDAASVASELFCQPY | B1162-116 | pNSB1280 | |
| A-8 | RRALRHCTGCMLSQRLGTAL | B1162-8 | pNSB1258 | A-117 | ARMALSLREWEYLFFKDAPSGPGL | B1162-117 | pNSB1228 | |
| A-9 | HSMHSCSVGRCLVDVKVVVS | B1162-9 | pNSB1231 | QGLSLASRLNLVILRGYG | ||||
| A-12 | WMVCAGCGALRTRQVTLHPG | B1162-12 | pNSB1232 | A-119 | RSYGGGEIPSVTMHCWIHCD | B1162-119 | pNSB1281 | |
| A-15 | GGFVPMRLCTCLLIVRLFI | B1162-15 | pNSB1264 | A-123 | SSSRWVPFALQDPLFSSDDW | B1162-123 | pNSB1282 | |
| A-16 | VPQPLNCDLCVLMGGASSSR | B1162-16 | pNSB1233 | A-124 | YLWSSKMDEWVAMDDVYAAC | B1162-124 | pNSB1283 | |
| A-18 | RRDYRKFFALNCQLCRLTVT | B1162-18 | pNSB1234 | A-127 | TWGLVCTGTGWGLLDTVVRA | B1162-127 | pNSB1284 | |
| A-22 | CRTRGCGCHLCRMLSQFTGG | B1162-22 | pNSB1235 | A-129 | VYEWGDVLCGGSMAIQWGL | B1162-129 | pNSB1254 | |
| A-25 | MRLGKGWNLMFLEEVSVLDA | B1162-25 | pNSB1323 | A-130 | ASNGEIAYCVEQAMLLLCFH | B1162-130 | pNSB1285 | |
| A-27 | RDPQLGQVAQTWGCRLCLLE | B1162-27 | pNSB1259 | A-131 | ELIVHEWPLILSRVGRIVL | B1162-131 | pNSB1255 | |
| A-30 | LVSESCGSWFCLCPWEVLNW | B1162-30 | pNSB1263 | A-132 | GRVQLEILVSEAEEGVSPFL | B1162-132 | pNSB1286 | |
| A-40 | LQYSWNLYSVASFKTRRVSS | B1162-40 | pNSB1236 | A-135 | RDAEWQDVLGRARAVHLRGR | B1162-135 | pNSB1287 | |
| A-41 | RLQESSIDLTPGIYLGMDFV | B1162-41 | pNSB1265 | A-136 | GLKWKSDNGCVYVSFMRGGV | B1162-136 | pNSB1288 | |
| A-46 | CYMEVEGRPRRWADSFFVAW | B1162-46 | pNSB1262 | A-137 | SSSPVPYSGGTCNLCSMRMW | B1162-137 | pNSB1289 | |
| A-49 | SESFVCKTCHMLRVSDAVGA | B1162-49 | pNSB1260 | A-140 | EWEDPQYAGWELFSISDLVH | B1162-140 | pNSB1256 | |
| A-50 | MHVSLVFPWRLTGHIQQYKV | B1162-50 | pNSB1261 | A-141 | PMVRTEWPLCAIIPLSMLYQ | B1162-141 | pNSB1290 | |
| A-51 | GRCNLQGMSFMGVGRSVWFE | B1162-51 | pNSB1237 | A-143 | RAGWHERVRQWWAIECTLEV | B1162-143 | pNSB1291 | |
| A-53 | VVGGSLRDEWKWWREGRSLP | B1162-53 | pNSB1267 | A-145 | SVRCWYVLRCSFLVGSGSSV | B1162-145 | pNSB1292 | |
| A-59 | AKDVERGAGGKIKACELCRL | B1162-59 | pNSB1268 | A-146 | RSCVLCAYGSRTFNGSYLLF | B1162-146 | pNSB1257 | |
| A-63 | VETFKARARQTPSCDLCPKT | B1162-63 | pNSB1238 | A-147 | GRGGCMLCDVDGSSAWLHTEGRLT | B1162-147 | pNSB1266 | |
| A-64 | TELWWADFAKMHMEGGKGMC | B1162-64 | pNSB1239 | GPITSQQCLSFQYLGNGEFIDG | ||||
| A-67 | RHRCTSRAPRQWFRPHRDSP | B1162-67 | pNSB1269 | A-149 | TLETLDMGNPLYTCVLMDWM | B1162-149 | pNSB1324 | |
| A-69 | RYRVSAGPLCSLCSLWGSVG | B1162-69 | pNSB1240 | A-150 | LVMGWRSEVSSLQGKTGTGGGPTL | B1162-150 | pNSB1293 | |
| A-71 | EEGLAAITHTWLTMCFAAGL | B1162-71 | pNSB1241 | RKCQLCRGSRYTLKYYPC | ||||
| A-73 | AAFLESVRSYWSRFVRHVQG | B1162-73 | pNSB1242 | A-153 | RPGCPFCTSWRCG | B1162-153 | pNSB1294 | |
| A-75 | RAMCDKDKSVCSILALYVQV | B1162-75 | pNSB1243 | A-155 | FCPECQMVAGAEDGDAIDLQ | B1162-155 | pNSB1295 | |
| A-80 | CWWLREIGTFRCVTLQHVAG | B1162-80 | pNSB1244 | A-158 | RRCMLCTSDKPGGDQGALNM | B1162-158 | pNSB1296 | |
| A-82 | FESAWSTLMGAMTPMVLDET | B1162-82 | pNSB1270 | A-159 | LWGGGTAWDFFVWGEDSAC | B1162-159 | pNSB1297 | |
| A-83 | QALVVSPETFLCLEALGVNS | B1162-83 | pNSB1271 | A-160 | GMSGRIPEPDDWVVLFITGC | B1162-160 | pNSB1298 | |
| A-84 | GGRQTEPSLTLLADLTLLLS | B1162-84 | pNSB1272 | A-161 | GGTNALLQKVFFGEVGVASM | B1162-161 | pNSB1299 | |
| A-86 | GSRAELSAPEVAWLLFCTPG | B1162-86 | pNSB1245 | A-164 | ECCLFPIFAMADSFPCPSPV | B1162-164 | pNSB1300 | |
| A-87 | RYSAVCRDCYEGHGRGLWYM | B1162-87 | pNSB1273 | A-165 | MLEGPLDQGLMMGTCCWECS | B1162-165 | pNSB1301 | |
| A-89 | GGWLVTIVEGPLAICCLRDD | B1162-89 | pNSB1274 | A-166 | TPSVTWLAEWCSCVFCRDAS | B1162-166 | pNSB1302 | |
| A-90 | PSIESGWVGDQAVAPCDLSV | B1162-90 | pNSB1275 | A-167 | SWWWANNSLCREWEFAC | B1162-167 | pNSB1303 | |
| A-91 | TWGAWKRDIVLVSEIGFTWG | B1162-91 | pNSB1246 | A-168 | WNMLAFGGALVASGLLRGWE | B1162-168 | pNSB1304 | |
| A-94 | RLGGGRPKLWHFSPNLMAGF | B1162-94 | pNSB1247 | A-169 | DKCDDVEPFLWWGQQCFFDV | B1162-169 | pNSB1305 | |
| A-97 | ERVHVCFSRKCTALSVDSSV | B1162-97 | pNSB1248 | A-170 | GSPSRISYTCLSPDVTLLFL | B1162-170 | pNSB1306 | |
| A-99 | RERGGDDYRRMMHPGAASGP | B1162-99 | pNSB1249 | A-172 | MGIEACSITECTSQHCNEVA | B1162-172 | pNSB1307 | |
| A-100 | RLVVGCEWRIGCSTGSGPRG | B1162-100 | pNSB1250 | A-173 | CLDNLCWELGGGFPVILIHC | B1162-173 | pNSB1308 | |
| A-101 | ASLIGVGIASMHGMQTDGIY | B1162-101 | pNSB1251 | A-174 | HVHGSCPSMGWSSNSWCSVF | B1162-174 | pNSB1309 | |
| A-108 | VGLMEWAVWSLEVREKLYSC | B1162-108 | pNSB1276 | A-175 | PLELEFAVCGCSWLVALDWS | B1162-175 | pNSB1310 | |
| A-109 | VLGRLGGAGGCSLCDQLEAL | B1162-109 | pNSB1277 | A-176 | AWDSESLATWASVMPWPYPT | B1162-176 | pNSB1311 | |
| A-110 | IWINPNGLWWTKVGLNPYAV | B1162-110 | pNSB1252 | A-177 | TGCHYKGARCCRLTWDVLIL | B1162-177 | pNSB1312 | |
| A-112 | RHESALHKSCELCYCPWKVC | B1162-112 | pNSB1278 |
a
The yeast plasmids were cloned into pJM-1 with carbenicillin selection.
b
The plant expression cassettes were cloned into pNSB1226 with kanamycin selection.